Third-generation HIV-1-derived lentiviral vectors are efficiently used as therapeutic brokers in varied scientific functions. To additional promote their use, we tried to reinforce vector infectivity by concentrating on the dimerization and packaging properties of the RNA switch vector based mostly on the premise that these two processes are tightly linked.
We rationally designed mutant vectors to favor the dimeric conformation, doubtlessly enhancing genome packaging. Preliminary assessments utilizing commonplace assays generated outputs of variable reproducibility, typically with conflicting outcomes. Due to this fact, we developed a novel aggressive qRT-PCR assay in a co-transfection setting to measure the relative packaging efficiencies of wild-type and mutant switch vectors.
Right here we report the impact of the dimerization-stabilizing mutations on infectious and bodily titers of lentiviral vectors along with their packaging effectivity, measured utilizing our novel assay. Enhancing dimerization didn’t robotically result in higher vector RNA packaging, suggesting that, for vector performance, adequate flexibility of the RNA to undertake totally different conformations is extra vital than the dimerization capability.
Our novel aggressive qPCR assay permits a extra stringent evaluation of RNA packaging effectivity, permitting a way more exact understanding of the hyperlinks between RNA construction, packaging, and infectious titers that will probably be invaluable for future vector improvement. To assemble RNA interference (RNAi) lentiviral expression vector of DEK gene, and to discover its impact on the organic habits of liver most cancers cells.
Double-stranded oligo DNAs have been annealed and synthesized in line with the interference sequence of DEK gene by RNAi know-how. Small interfering RNA expression vector pLKO.1 was cloned after enzymatic digestion. The recombinant lentiviral pLKO.1-sh hDEK was constructed, after which the virus supernatant was collected, packed and contaminated by 293T cells.
Manufacturing of lentiviral vectors in suspension cells utilizing low proportion of supercoiled round plasmid DNA
The supercoiled round (SC) topology type of plasmid DNA has been regarded to be advantageous over open round or linearized analogue in transfection and expression effectivity, and due to this fact are largely demanded within the biopharmaceutical manufacturing. Nevertheless, manufacturing of high-purity SC plasmid DNA would end in excessive manufacturing value.
The impact of SC proportion in plasmid DNA on the standard of packaged lentiviral vectors has by no means been reported. On this research, we established an environment friendly system for manufacturing of high-titer lentiviral vectors utilizing suspension HEK293SF cells in serum-free media, and the lentiviral titer was not related to the proportion of SC plasmid DNA.
Plasmids DNA with totally different proportion of SC, open-circular, and linearized kinds have been ready utilizing the thermal denaturation methodology, and have been transfected to adherent HEK293T or suspension HEK293SF cells for packaging of lentiviral vectors. The titer of lentiviral vectors from HEK293T cells, however not from HEK293SF cells, was considerably impaired when the proportion of SC plasmid DNA decreased from 60-80% to 30-40%.
Additional lower of SC plasmid proportion to three% led to a dramatic discount of lentiviral titer regardless of the packaging cell line was. Nevertheless, lentiviral vectors from HEK293SF cells nonetheless confirmed a excessive titer even when the proportion of SC plasmid DNA was 3%. This research demonstrated that extraordinarily excessive proportion of SC plasmid DNA was not required for packaging of high-titer lentiviral vector in HEK293SF cells, no less than underneath our manufacturing course of.
WAS Promoter-Pushed Lentiviral Vectors Mimic Intently the Lopsided WASP Expression throughout Megakaryocytic Differentiation
Transplant of gene-modified autologous hematopoietic progenitors cells has emerged as a brand new therapeutic method for Wiskott-Aldrich syndrome (WAS), a major immunodeficiency with microthrombocytopenia and irregular lymphoid and myeloid features. Regardless of the scientific advantages obtained in ongoing scientific trials, platelet restoration is suboptimal.
The unfinished restoration of platelets in these sufferers might be defined both by a low variety of corrected cells or by inadequate or insufficient WASP expression throughout megakaryocyte differentiation and/or in platelets. We due to this fact used in vitro fashions to review the endogenous WASP expression sample throughout megakaryocytic differentiation and in contrast it with the expression profiles achieved by totally different therapeutic lentiviral vectors (LVs) driving WAS cDNA by means of totally different areas of the WAS promoter.
Our knowledge confirmed that every one WAS promoter-driven LVs mimic very intently the endogenous WAS expression kinetic throughout megakaryocytic differentiation. Nevertheless, LVs harboring the full-length (1.6-kb) WAS-proximal promoter (WW1.6) or a mix of the WAS various and proximal promoters (named AW) had the most effective habits. Lastly, all WAS-driven LVs restored the WAS knockout (WASKO) mice phenotype and purposeful defects of hematopoietic stem and progenitor cells (HSPCs) from a WAS affected person with related effectivity.
C1orf132 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720027 | ABM | 1.0 ug DNA | Ask for price |
C1orf134 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV720029 | ABM | 1.0 ug DNA | Ask for price |
C1orf134 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720033 | ABM | 1.0 ug DNA | Ask for price |
C1orf138 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV720035 | ABM | 1.0 ug DNA | Ask for price |
C1orf138 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720039 | ABM | 1.0 ug DNA | Ask for price |
C1orf145 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV720041 | ABM | 1.0 ug DNA | Ask for price |
C1orf145 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720045 | ABM | 1.0 ug DNA | Ask for price |
C1orf148 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV720047 | ABM | 1.0 ug DNA | Ask for price |
C1orf148 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720051 | ABM | 1.0 ug DNA | Ask for price |
C1orf195 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV720053 | ABM | 1.0 ug DNA | Ask for price |
C1orf195 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720057 | ABM | 1.0 ug DNA | Ask for price |
C1orf196 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV720059 | ABM | 1.0 ug DNA | Ask for price |
C1orf196 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720063 | ABM | 1.0 ug DNA | Ask for price |
C6orf100 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV720113 | ABM | 1.0 ug DNA | Ask for price |
C6orf100 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720117 | ABM | 1.0 ug DNA | Ask for price |
C6orf183 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV720119 | ABM | 1.0 ug DNA | Ask for price |
C6orf183 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720123 | ABM | 1.0 ug DNA | Ask for price |
C9orf107 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV720155 | ABM | 1.0 ug DNA | Ask for price |
C9orf107 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720159 | ABM | 1.0 ug DNA | Ask for price |
C9orf118 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV720161 | ABM | 1.0 ug DNA | Ask for price |
C9orf118 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720165 | ABM | 1.0 ug DNA | Ask for price |
C9orf141 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV720167 | ABM | 1.0 ug DNA | Ask for price |
C9orf141 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720171 | ABM | 1.0 ug DNA | Ask for price |
C1orf222 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV701565 | ABM | 1.0 ug DNA | EUR 540 |
C1orf222 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV701569 | ABM | 1.0 ug DNA | EUR 540 |
C1orf126 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV706029 | ABM | 1.0 ug DNA | EUR 540 |
C1orf126 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV706033 | ABM | 1.0 ug DNA | EUR 540 |
C1orf212 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV706299 | ABM | 1.0 ug DNA | EUR 540 |
C1orf212 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV706303 | ABM | 1.0 ug DNA | EUR 540 |
C1orf109 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV707955 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf109 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV707959 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf116 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV707961 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf116 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV707965 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf124 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV707967 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf124 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV707971 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf137 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV758165 | ABM | 1.0 ug DNA | Ask for price |
C1orf137 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV758169 | ABM | 1.0 ug DNA | Ask for price |
C1orf143 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV758171 | ABM | 1.0 ug DNA | Ask for price |
C1orf143 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV758175 | ABM | 1.0 ug DNA | Ask for price |
C1ORF233 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV758177 | ABM | 1.0 ug DNA | Ask for price |
C1ORF233 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV758181 | ABM | 1.0 ug DNA | Ask for price |
C6ORF228 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV758219 | ABM | 1.0 ug DNA | EUR 547.2 |
C6ORF228 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV758223 | ABM | 1.0 ug DNA | EUR 547.2 |
C1orf149 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV809829 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf149 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV809833 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf105 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV809883 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf105 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV809887 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf107 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV810021 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf107 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV810025 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf217 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV810033 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf217 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV810037 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf163 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV810195 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf163 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV810199 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf211 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV810231 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf211 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV810235 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf218 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV810243 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf218 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV810247 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf182 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV810465 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf182 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV810469 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf168 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV810681 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf168 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV810685 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf142 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV810861 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf142 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV810865 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf190 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV810897 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf190 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV810901 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf102 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV811083 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf102 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV811087 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf156 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV811335 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf156 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV811339 | ABM | 1.0 ug DNA | EUR 379.2 |
C9orf125 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV811425 | ABM | 1.0 ug DNA | EUR 379.2 |
C9orf125 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV811429 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf168 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV811449 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf168 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV811453 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf134 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV812229 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf134 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV812233 | ABM | 1.0 ug DNA | EUR 379.2 |
C9orf102 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV812337 | ABM | 1.0 ug DNA | EUR 379.2 |
C9orf102 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV812341 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf146 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV812415 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf146 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV812419 | ABM | 1.0 ug DNA | EUR 379.2 |
C9orf150 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV812571 | ABM | 1.0 ug DNA | EUR 379.2 |
C9orf150 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV812575 | ABM | 1.0 ug DNA | EUR 379.2 |
C9orf171 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV791197 | ABM | 1.0 ug DNA | EUR 540 |
C1orf227 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV795069 | ABM | 1.0 ug DNA | EUR 547.2 |
C6orf134 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV812769 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf134 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV812773 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf182 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV812931 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf182 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV812935 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf157 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV814125 | ABM | 1.0 ug DNA | EUR 540 |
C1orf157 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV814129 | ABM | 1.0 ug DNA | EUR 540 |
C1orf175 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV814209 | ABM | 1.0 ug DNA | EUR 540 |
C1orf175 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV814213 | ABM | 1.0 ug DNA | EUR 540 |
C6orf190 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV814239 | ABM | 1.0 ug DNA | EUR 540 |
C6orf190 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV814243 | ABM | 1.0 ug DNA | EUR 540 |
C6orf184 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV814341 | ABM | 1.0 ug DNA | EUR 540 |
C6orf184 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV814345 | ABM | 1.0 ug DNA | EUR 540 |
C6orf185 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV814485 | ABM | 1.0 ug DNA | EUR 540 |
C6orf185 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV814489 | ABM | 1.0 ug DNA | EUR 540 |
C6orf150 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV815067 | ABM | 1.0 ug DNA | EUR 540 |
C6orf150 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV815071 | ABM | 1.0 ug DNA | EUR 540 |
C9orf127 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV815229 | ABM | 1.0 ug DNA | EUR 540 |
C9orf127 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV815233 | ABM | 1.0 ug DNA | EUR 540 |
C6orf122 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV815247 | ABM | 1.0 ug DNA | EUR 540 |
C6orf122 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV815251 | ABM | 1.0 ug DNA | EUR 540 |
C1orf161 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV815451 | ABM | 1.0 ug DNA | EUR 540 |
C1orf161 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV815455 | ABM | 1.0 ug DNA | EUR 540 |
C1orf104 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV815475 | ABM | 1.0 ug DNA | EUR 540 |
C1orf104 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV815479 | ABM | 1.0 ug DNA | EUR 540 |
C1orf104 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV815511 | ABM | 1.0 ug DNA | EUR 540 |
C1orf104 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV815515 | ABM | 1.0 ug DNA | EUR 540 |
C1orf128 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV809193 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf142 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV809265 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf142 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV809269 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf183 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV809343 | ABM | 1.0 ug DNA | EUR 379.2 |
C1orf103 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV809487 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf115 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV809673 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf115 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV809677 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf125 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV809763 | ABM | 1.0 ug DNA | EUR 379.2 |
C6orf125 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV809767 | ABM | 1.0 ug DNA | EUR 379.2 |
C10orf100 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV720173 | ABM | 1.0 ug DNA | Ask for price |
C10orf100 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV720177 | ABM | 1.0 ug DNA | Ask for price |
C10orf101 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV720179 | ABM | 1.0 ug DNA | Ask for price |
C10orf101 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV720183 | ABM | 1.0 ug DNA | Ask for price |
C10orf102 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV720185 | ABM | 1.0 ug DNA | Ask for price |
C10orf102 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV720189 | ABM | 1.0 ug DNA | Ask for price |
C14orf144 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV720233 | ABM | 1.0 ug DNA | Ask for price |
C14orf144 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV720237 | ABM | 1.0 ug DNA | Ask for price |
C14orf164 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV720239 | ABM | 1.0 ug DNA | Ask for price |
C14orf164 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV720243 | ABM | 1.0 ug DNA | Ask for price |
C20orf168 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV720317 | ABM | 1.0 ug DNA | Ask for price |
C20orf168 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV720321 | ABM | 1.0 ug DNA | Ask for price |
C20orf187 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV720323 | ABM | 1.0 ug DNA | Ask for price |
C20orf187 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720327 | ABM | 1.0 ug DNA | Ask for price |
C21orf118 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV720347 | ABM | 1.0 ug DNA | Ask for price |
C21orf118 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV720351 | ABM | 1.0 ug DNA | Ask for price |
C20orf132 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV704727 | ABM | 1.0 ug DNA | EUR 540 |
C20orf132 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV704731 | ABM | 1.0 ug DNA | EUR 540 |
C10orf118 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV702213 | ABM | 1.0 ug DNA | EUR 540 |
C10orf118 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV702217 | ABM | 1.0 ug DNA | EUR 540 |
In abstract, our knowledge again up using WW1.6 and AW LVs as physiological gene switch instruments for WAS remedy. Traditionally, environment friendly transduction of hematopoietic stem cells (HSC) to review the function of particular genes on HSC perform, in addition to to broaden the potential of gene remedy for hematopoietic associated illnesses has relied on our capacity to design vectors able to delivering the gene of curiosity with out affecting HSC perform.